Identification and Analysis of Ku70 and Ku80 Homologs in the koji Molds Aspergillus sojae and Aspergillus oryzae
<I>Ku</I> genes play a key role in the non-homologous end-joining pathway. We have identified <I>Ku70</I> and <I>Ku80</I> homologs in the <I>koji</I> molds <I>Aspergillus sojae</I> and <I>Aspergillus oryzae</I>, and have constructed the disruption mutants of <I>Ku70</I>, <I>Ku80</I>, and <I>Ku70–80</I> to characterize the phenotypic change in these mutants. Neither <I>Ku70</I>- nor <I>Ku80</I>-disrupted strains show hypersensitivity to the DNA damaging agents methylmethane sulfonate (MMS) and phleomycin. Moreover, undesirable phenotypes, such as poor growth or repressed conidiospore formation, were not observed in the <I>Ku</I>-disrupted <I>A. sojae</I> and <I>A. oryzae</I>.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(1), 135-143, 2006-01-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry