Identification and Analysis of Ku70 and Ku80 Homologs in the koji Molds Aspergillus sojae and Aspergillus oryzae

• TAKAHASHI Tadashi

  Noda Institute for Scientific Research

• MASUDA Tsutomu

  Noda Institute for Scientific Research

• KOYAMA Yasuji

  Noda Institute for Scientific Research

Bibliographic Information

Other Title

• Identification and Analysis of<i>Ku70</i>and<i>Ku80</i>Homologs in the<i>koji</i>Molds<i>Aspergillus sojae</i>and<i>Aspergillus oryzae</i>
• Identification and analysis of <italic>Ku70</italic> and <italic>Ku80</italic> homologs in the <italic>koji</italic> molds <italic>Aspergillus sojae</italic> and <italic>Aspergillus oryzae</italic>

Abstract

Ku genes play a key role in the non-homologous end-joining pathway. We have identified Ku70 and Ku80 homologs in the koji molds Aspergillus sojae and Aspergillus oryzae, and have constructed the disruption mutants of Ku70, Ku80, and Ku70–80 to characterize the phenotypic change in these mutants. Neither Ku70- nor Ku80-disrupted strains show hypersensitivity to the DNA damaging agents methylmethane sulfonate (MMS) and phleomycin. Moreover, undesirable phenotypes, such as poor growth or repressed conidiospore formation, were not observed in the Ku-disrupted A. sojae and A. oryzae.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 70 (1), 135-143, 2006

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• non-homologous end-joining
• Ku70
• Ku80

Details

• CRID: 1390001206475500928
• NII Article ID: 10018535311
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.70.135
• COI: 1:CAS:528:DC%2BD28XhsVGmtLY%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 7791458
• PubMed: 16428831
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7791458
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.135
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed