Effect of Bombyx mori Chitinase against Japanese Pine Sawyer (Monochamus alternatus) Adults as a Biopesticide

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<I>Bombyx mori</I> chitinase (<I>Bm</I>-CHI), with a molecular mass of 75 kDa, was investigated on the possibility that it can serve as a biocontrol agent against the adult Japanese pine sawyer (JPS), <I>Monochamus alternatus</I> (Coleoptera: Cerambycidae). Oral ingestion of purified chitinase at concentrations of 3 μ<small>M</small> (11.25 μg/50 μl) and 0.3 μ<small>M</small> (1.125 μg/50 μl) caused high mortality in JPS, a significant decrease in bark consumption, and, only in high concentration, a slight reduction of body weight. Fluorescence assays indicated that peritrophic membrane (PM) chitin is degraded by the action of orally ingested <I>Bm</I>-CHI at 3 μ<small>M</small> concentration only. Scanning electron micrographs clearly indicated that the beetles that ingested <I>Bm</I>-CHI of the same high concentration had their PM perforated and disrupted, but ultrastructural studies showed that the ingested chitinase did not affect the midgut epithelium. These findings open up the possibility of using insect chitinase as a biopesticidal enzyme. It should have agronomic potential for insect control.

収録刊行物

  • Bioscience, biotechnology, and biochemistry  

    Bioscience, biotechnology, and biochemistry 70(1), 219-229, 2006-01-23 

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

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各種コード

  • NII論文ID(NAID)
    10018535556
  • NII書誌ID(NCID)
    AA10824164
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    09168451
  • NDL 記事登録ID
    7791660
  • NDL 雑誌分類
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL 請求記号
    Z53-G223
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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