A Simple Method with Liquid Chromatography/Tandem Mass Spectrometry for the Determination of the Six Trichothecene Mycotoxins in Rice Medium
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A selective and speedy LC–MS/MS method was developed to determine six trichothecene mycotoxins (nivalenol, deoxynivalenol, fusarenon X, 15-acetyldeoxynivalenol, 3-acetyldeoxynivalenol, and T-2 toxin) in rice medium where <I>Fusarium graminearum</I> were cultivated for <I>in vitro</I> tests. The analytes were extracted from the rice medium with acetonitrile/water (85/15, v/v), and diluted with acetonitrile/water (5/95, v/v) in order to minimize the effects of matrices. Diluted solutions were analyzed by LC–MS/MS with electrospray ionization (ESI) interface in negative or positive ion mode and the multiple reaction monitoring mode. Recovery rates were 76–106% with a spiked level at 1–6 μg/kg of mycotoxins that corresponded to the limit of quantitation. The method was applied to study the time courses of trichothecene production and the biomass of fungi by three <I>Fusarium graminearum</I> strains. Three strains have different mycotoxin biosynthesis pathways, wFg14 and 03E-1 were DON producer, and 03N-1 was NIV producer.
- Agricultural and Biological Chemistry
Agricultural and Biological Chemistry 70(1), 230-236, 2006-01-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry