Identification of sn-Glycerol-1-phosphate Dehydrogenase Activity from Genomic Information on a Hyperthermophilic Archaeon, Sulfolobus tokodaii Strain 7
<I>sn</I>-Glycerol-1-phosphate dehydrogenase is responsible for the formation of <I>sn</I>-glycerol-1-phosphate, the backbone of membrane phospholipids of Archaea. This activity had never been detected in cell-free extract of <I>Sulfolobus</I> sp. Here we report the detection of this activity on the thermostable ST0344 protein of <I>Sulfolobus tokodaii</I> expressed in <I>Escherichia coli</I>, which was predicted from genomic information on <I>S. tokodaii</I>. This is another line of evidence for the general mechanism of <I>sn</I>-glycerol-1-phosphate formation by the enzyme.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(1), 282-285, 2006-01-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry