Atomic Force Microscopic Observation of<i>Escherichia coli</i>Ribosomes in Solution

• MATSUURA Toshihiko

  Institute of Scientific and Industrial Research, Osaka University Technology Research Laboratory, Shimadzu Corporation

• TANAKA Hiroyuki

  Institute of Scientific and Industrial Research, Osaka University

• MATSUMOTO Takuya

  Institute of Scientific and Industrial Research, Osaka University

• KAWAI Tomoji

  Institute of Scientific and Industrial Research, Osaka University

書誌事項

タイトル別名

• Atomic Force Microscopic Observation of Escherichia coli Ribosomes in Solution

抄録

Ribosomes of Escherichia coli were visualized in buffer solution by atomic force microscopy (AFM). A series of time-dependent AFM images showed that ribosomes spontaneously adsorb on mica. Although ribosomes observed in air are forced to flatten on the surface, the height of ribosomal particles obtained under a physiological buffer solution is 21.8±0.5 nm, which is consistent with the ideal diameter. We succeeded in observing single ribosomes in a near-native condition.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 70 (1), 300-302, 2006

  公益社団法人 日本農芸化学会

キーワード

• <I>Escherichia coli</I> ribosome
• bioimaging
• adsorption
• atomic force microscopy (AFM)

詳細情報

• CRID: 1390001206475540608
• NII論文ID: 10018535893
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.70.300
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 7792100
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7792100
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.70.300
• 本文言語コード: en
• データソース種別:
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  • NDL
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• 抄録ライセンスフラグ: 使用不可