Atomic Force Microscopic Observation of Escherichia coli Ribosomes in Solution
Ribosomes of <I>Escherichia coli</I> were visualized in buffer solution by atomic force microscopy (AFM). A series of time-dependent AFM images showed that ribosomes spontaneously adsorb on mica. Although ribosomes observed in air are forced to flatten on the surface, the height of ribosomal particles obtained under a physiological buffer solution is 21.8±0.5 nm, which is consistent with the ideal diameter. We succeeded in observing single ribosomes in a near-native condition.
- Bioscience, biotechnology, and biochemistry
Bioscience, biotechnology, and biochemistry 70(1), 300-302, 2006-01-23
Japan Society for Bioscience, Biotechnology, and Agrochemistry