鼻茸における好酸球浸潤と好酸球遊走因子および関連因子 : アトピー素因の有無による検討 Eosinophil Chemoattractants and Related Factors in Nasal Polyps
〔目的〕慢性副鼻腔炎患者の鼻茸を用いて,免疫染色および酵素免疫測定法(ELISA法)によって好酸球遊走因子と関連因子を同定,定量し,アトピー素因の有無により解析し,鼻茸組織中の好酸球の浸潤機序を検証した.<br>〔対象と方法〕鼻茸を伴う慢性副鼻腔炎20症例より摘出した鼻茸ホモジネート中のinterleukin(IL-)5,IL-13,eotaxin,thymus and activation regulated chemokine(TARC),regulated upon activation nomal T cell expressed and secreted(RANTES)をELISA法で定量した.また同一症例での鼻茸組織切片の好酸球数およびEG2陽性細胞数をカウントした.<br>〔結果〕アトピー素因の有無で鼻茸組織中の好酸球数,EG2陽性細胞数および鼻茸ホモジネート中のIL-5,eotaxin,TARC,RANTESの濃度に有意な差を認めなかった.同一症例においての組織中好酸球数とIL-5濃度,eotaxin濃度,TARC濃度との間に相関を認めたが,好酸球数とRANTES濃度との間には相関を認めなかった.IL-13はすべての症例で検出限界値以下であった.<br>〔結論〕鼻茸組織への好酸球浸潤はアトピー素因の有無に関わらずIL-5,eotaxin,TARCの関与が考えられた.
Objective: In vitro studies and animal experiments have shown that cytokines and chemokines are closely related to eosinophil migration, activation, and survival. It remains controversial, however, whether some chemokines or cytokines are actually responsible for the accumulation of eosinophils in nasal polyp tissues. We studied cytokines and chemokines in nasal polyp tissues taken from patients with chronic rhinosinusitis to clarify the pathogenesis of eosinophil accumulation.<br>Materials and methods: Nasal polyp tissues obtained from 20 patients with chronic rhinosinusitis were studied. Concentrations of interleukin (IL-)5, IL-13. eotaxin, regulated upon activation in normal T cell expressed and secreted (RANTES). and thymus and activation-regulated chemokine (TARC) in homogenates of polyp tissues were measured by ELISA. Nasal polyp tissues were stained by hematoxillin and eosin and were immunostained by an antibody against EG2. The numbers of cosinophils and immunopositive cells for EG2 in the submuicosal layer were counted using a microscope.<br>Results: No significant differences were seen in the numbers of eosinophils and EG2-positive cells, or in the concentration of IL-5, eotaxin, TARC, RANTES in nasal polyp tissues between patients with and without atopic predisposition. Significant positive correlations existed, however, between the number of eosinophils and IL-5, eotaxin, and TARC concentration. IL-13, concentration was below detection in all patients.<br>Conclusion: We hound that IL-5, eotaxin, and TARC may play an important role in the accumulation of eosinophils in nasal polyps regardless of the presence of atopic predisposition.
日本耳鼻咽喉科學會會報 109(11), 774-780, 2006-11-20
The Oto-Rhino-Laryngological Society of Japan, Inc.