Post-transcriptional Regulation of Chicken ccn2 Gene by Interaction between RNA cis-element and trans-factor during Differentiation of Chondrocytes

• Mukudai Yoshiki

  Orthopaedic Surgery, Washington University School of Medicine

抄録

CCN2/CTGF is a multifunctional growth factor. Previous studies have revealed that CCN2 plays important roles in the growth and differentiation of chondrocytes, and that the 3’-untranslated region (3’-UTR) of ccn2 mRNA contains a cis-repressive element of gene expression. In the present review, it is shown that chicken ccn2 mRNA is post-transcriptionally regulated depending not only on the differentiation stages of chondrocytes, but also on the stimulation of growth factors. In addition, the minimal repressive cis-element of the 3’-UTR of chicken ccn2 mRNA was located within an area between 50 and 100 bases from the polyadenylation tail. Moreover, the stability of ccn2 mRNA was correlated with the interaction between the cis-element and a 40-kDa-putative-shuttle-trans-factor in the nuclei and cytoplasm. In summary, these results suggest that gene expression of ccn2 mRNA during endochondral ossification is properly regulated, at least in part, by the changing stability of the mRNA, which arises from the interaction between the RNA cis-element and putative trans-factor.

収録刊行物

• Journal of Oral Biosciences

  Journal of Oral Biosciences 49 (1), 15-22, 2007

  歯科基礎医学会

キーワード

• mRNA stability
• 3’-untranslated region
• CCN family
• chondrocytes
• endochondral ossification

詳細情報

• CRID: 1390001205213435392
• NII論文ID: 130004475632
• NII論文ID: 10018562789
• NII書誌ID: AA11896386
• DOI: 10.2330/joralbiosci.49.15
• ISSN: 18803865
• ISSN: 13490079
• Web Site: https://search.jamas.or.jp/link/ui/2007281426
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可