Experimentally manipulating fungi with optical tweezers

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  • Wright Graham D.
    Fungal Cell Biology Group, Institute of Cell Biology, University of Edinburgh Collaborative Optical Spectroscopy, Micromanipulation and Imaging Centre (COSMIC), University of Edinburgh
  • Arlt Jochen
    Collaborative Optical Spectroscopy, Micromanipulation and Imaging Centre (COSMIC), University of Edinburgh SUPA and School of Physics, University of Edinburgh
  • Poon Wilson C.K.
    Collaborative Optical Spectroscopy, Micromanipulation and Imaging Centre (COSMIC), University of Edinburgh SUPA and School of Physics, University of Edinburgh
  • Read Nick D.
    Fungal Cell Biology Group, Institute of Cell Biology, University of Edinburgh Collaborative Optical Spectroscopy, Micromanipulation and Imaging Centre (COSMIC), University of Edinburgh

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Abstract

<p>A short review of the use of optical tweezers in fungal cell biological research is provided. First, we describe how optical tweezers work. Second, we review how they have been used in various experimental live-cell studies to manipulate intracellular organelles, hyphal growth and branching, and whole cells. Third, we indicate how optically trapped microbeads can be used for the localized delivery of chemicals or mechanical stimulation to cells, as well as permitting measurements of the growth forces generated by germ tubes. Finally, the effects of optical trapping on fungal cell viability and growth are assessed.</p>

Journal

  • Mycoscience

    Mycoscience 48 (1), 15-19, 2007

    The Mycological Society of Japan

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