Quantitative Analysis of Active Oxygen Species by Potentiometry

• YUASA Makoto

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science Institute of Colloid and Interface Science, Tokyo University of Science

• OYAIZU Kenichi

  Institute of Colloid and Interface Science, Tokyo University of Science

• MURATA Hidenori

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science

• EGUCHI Katsuya

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science

• TOYODA Yujiro

  Department of Pure and Applied Chemistry, Faculty of Science and Engineering, Tokyo University of Science

Bibliographic Information

Other Title

• ポテンショメトリーによる活性酸素種の定量検出
• ポテンショメトリー ニ ヨル カッセイ サンソシュ ノ テイリョウ ケンシュツ

Abstract

Reactive oxygen species (ROS) have many vital functions including sterilizing function of white blood cell, carcinostatic function, aging, cell death, inflammation, and DNA damage. Among various ROS, superoxide anion radical (O₂^-•) has been attracting great attention as a cause of human disease. We have developed an O₂^-• sensor composed of a thin film of 1-methylimidazole(Im) coordinatied Bromoiron(III) meso-tetrathienyleporphyrin ([FeBr(ttp)]) for the electro-chemical detection. In this study, highly sensitive measurements of O₂^-• by potentiometry were conducted. The potentiometric response for O₂^-• was monitored in 10 mM phosphate buffer. The O₂^-• sensor displayed a high catalytic activity for oxidation of O₂^-• and showed a linear relationship between the sensor response and the logarithm of O₂^-• concentration. This O₂^-• sensor showed low interferences due to the electrochemical interferents H₂O₂ and uric acid. Measurement of O₂^-• by potentiometry was able to detect the low concentrations of O₂^-• (7×10^-7 M) that measurements of O₂^-• by amperometry could not easily detect.<br>

Journal

• KOBUNSHI RONBUNSHU

  KOBUNSHI RONBUNSHU 64 (2), 90-95, 2007

  The Society of Polymer Science, Japan

Keywords

• Electrochemical Sensor
• Superoxide Anion Radical
• Ironporphyrin
• Potentiometry

Details

• CRID: 1390282681500095872
• NII Article ID: 10018576401
• NII Book ID: AN00085011
• DOI: 10.1295/koron.64.90
• COI: 1:CAS:528:DC%2BD2sXktVSnsLo%3D
• ISSN: 18815685
• ISSN: 03862186
• NDL BIB ID: 8726056
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8726056
• Web Site: https://www.jstage.jst.go.jp/article/koron/64/2/64_2_90/_pdf
• Text Lang: ja
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed