Separation of Alkaloids from Sophora alopecuroides L. by Adsorption Using Macroporous Resins
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A macroporous adsorption resin, DF01 (cross-linked polystyrene, particle size 0.3–1.0 mm, specific area 400–420 m<SUP>2</SUP> g<SUP>–1</SUP>), was selected from twelve kinds of resins and used to adsorb alkaloids from <I>Sophora alopecuroides</I> L. The adsorption capacity of DF01 on total alkaloids was as high as 17 mg cm<SUP>–3</SUP> at pH 10.0 and the NaCl concentration of 1.0 mol L<SUP>–1</SUP>. Alkaloids were desorbed by ethanol/water (80:20, v/v) of pH 3.0 and 96% of the adsorbed alkaloids were eluted. The alkaloid monomers were separated by gradient elution with ethanol/water solutions of different volume ratios at reduced pressure. The major alkaloid monomers were obtained with a purity of over 90%.
- JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN 40(2), 93-97, 2007-02-01
The Society of Chemical Engineers, Japan