唾液腺腺様嚢胞癌の増殖・進展メカニズムに関する研究 : NCAMの発現とその機能について A study of proliferation and progression in adenoid cystic carcinomas : Expression and functions of NCAM

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著者

    • 松原 完也 MATSUBARA Kanya
    • 明海大学歯学部口腔外科学第2講座 Second Department of Oral and Maxillofacial Surgery, Meikai University School of Dentistry
    • 福田 正勝 FUKUDA Masakatsu
    • 明海大学歯学部口腔外科学第2講座 Second Department of Oral and Maxillofacial Surgery, Meikai University School of Dentistry
    • 草間 薫 KUSAMA Kaoru
    • 明海大学歯学部口腔病理学講座 Department of Oral Pathology, Meikai University School of Dentistry
    • 坂下 英明 SAKASHITA Hideaki
    • 明海大学歯学部口腔外科学第2講座 Second Department of Oral and Maxillofacial Surgery, Meikai University School of Dentistry

抄録

Neural cell adhesion molecule (NCAM) is one cell surface glycoprotein and a member of the immunoglobulin superfamily. It has been reported that NCAM may be associated with perineural invasion by malignant glandular tumors, such as bile duct carcinoma or gallbladder carcinoma. However, it remains unclear whether NCAM is really associated with the development of salivary gland tumors. Previous studies have demonstrated that NCAM is constitutively expressed in the human salivary gland tumor cell line HSG in vitro. In this study, molecular mechanisms of perineural invasion were examined using HSG cells in which NCAM-mediated inhibition of salivary gland tumor proliferation is caused by homophilic binding. In addition, the localization of NCAM in adenoid cystic carcinomas (ACCs) was examined immunohistochemically. The levels of NCAM mRNA and protein expression were found to decrease in a dose-dependent manner on treatment with anti-NCAM antibody. The MTT assay revealed a significant reduction in the number of viable HSG cells. Confocal laser microscopy showed that HSG cells undergo apoptosis in response to treatment with anti-NCAM antibody. The activation of caspases 3, 7, and 9 was observed in HSG cells after treatment with anti-NCAM antibody, thus confirming the induction of activated caspase-mediated apoptosis. The up-regulation of TGF-β1-mediated NCAM expression seemed to lead to the activation of homophilic NCAM binding, further accelerating HSG cell proliferation. Immunohistochemical examinations revealed that NCAM was slightly to moderately positive in 9 of 13 cases (69.2%) of ACC.<BR>These findings suggest that NCAM is associated not only with a cell-to-cell adhesion mechanism but also with tumorigenesis, including growth, development, and perineural invasion in human ACCs.

収録刊行物

  • 日本口腔外科学会雑誌  

    日本口腔外科学会雑誌 50(12), 749-758, 2004-12-20 

    Japanese Society of Oral and Maxillofacial Surgeons

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各種コード

  • NII論文ID(NAID)
    10018618536
  • NII書誌ID(NCID)
    AN00189163
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215163
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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