口腔扁平上皮癌細胞の浸潤能に及ぼすets-1の影響 Effects of ets-1 on invasive properties of oral squamous carcinoma cells
Metastasis in invasive carcinoma is closely associated with the production of extracellular matrix catabolic enzymes such as matrix metalloproteinases (MMPs), as well as with enhanced cell motility and growth. It is known that <I>ets</I> oncogenes exist in transcriptional regulation regions of MMPs, such as AP1 sites, and enhance or inhibit MMP transcription. In this study, we investigated the effect of ets-1, a member of the ets oncogene family, on the invasive properties of oral cancer from a molecular biological perspective. By means of lipofection, an oral squamous carcinoma cell line was transfected with <I>ets</I>-1. The invasive ability of the transfected cells was determined using a matrigel invasion assay, and MMP-9 production was measured using gelatin zymography. The results for the transfected cells were compared with those for normal cultured cells. Matrigel invasion assay revealed that the transfected cells had higher invasive ability than that of normal cultured cells, and gelatin zymography demonstrated that their MMP-9 production had increased. These results suggest that ets-1 is involved in enhanced invasive ability and increased MMP-9 production in oral squamous carcinoma cells.
日本口腔外科学会雑誌 50(12), 759-764, 2004-12-20
Japanese Society of Oral and Maxillofacial Surgeons