骨髄細胞を用いた二次的血管柄付き移植骨片作製に関する実験的研究 Experimental study of prefabricated vascularized bone graft using bone marrow cells

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著者

    • 福田 あおい FUKUDA Aoi
    • 大阪歯科大学口腔外科学第1講座 First Department of Oral and Maxillofacial Surgery, Osaka Dental University
    • 森田 章介 MORITA Shosuke
    • 大阪歯科大学口腔外科学第1講座 First Department of Oral and Maxillofacial Surgery, Osaka Dental University

抄録

Vascularized bone grafts have been widely used to replace bone lost by disease or trauma. While this approach has been generally successful, some problems remain. Autologous bone grafts are limited in supply and can occasionally cause severe surgical trauma. Recently, some studies were undertaken to evaluate the potential of using cultured cells and biomaterials to produce tissue-engineered bone.<BR>We created prefabricated vascularized bone grafts in the femur of rats by the combined use of a collagen sponge scaffold, osteoblasts differentiated from rat bone marrow, and a vascular bundle in a diffusion chamber. In the experimental group, the saphenous vessels of 8-week-old Fischer rats were exposed under general anesthesia and placed on slits cut into the diffusion chamber. Then, a collagen sponge scaffold seeded with osteoblasts was packed into the diffusion chamber. In the control group, the procedure was similar, without the placement of vessels. One, 3, 6, and 9 weeks after operation, the implants were harvested, and new bone formation was assessed histologically and biochemically.<BR>In the experimental group, histological analysis showed active bone formation in the diffusion chamber. Bone formation was confirmed by the presence of osteoblasts lining the surface of new bone. Substantial alkaline phosphatase activity in the composites was detected soon after the procedure. Osteocalcin, detected immediately after implantation, increased slightly later. In contrast, there was no significant bone formation in the control group.<BR>These results suggest that the combination of a collagen scaffold, osteoblasts, and a vascular bundle in the chamber may be a useful method for the prefabrication of vascularized bone grafts.

収録刊行物

  • 日本口腔外科学会雑誌  

    日本口腔外科学会雑誌 50(10), 567-576, 2004-10-20 

    Japanese Society of Oral and Maxillofacial Surgeons

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各種コード

  • NII論文ID(NAID)
    10018618947
  • NII書誌ID(NCID)
    AN00189163
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215163
  • データ提供元
    CJP書誌  J-STAGE 
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