Detection of Lung Cancer Cells in Bronchial Washings Using Fluorescence in Situ Hybridization

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  • Aute Idiris
    Department of Surgery, Tokyo Medical University Department of Thoracic Surgery, First Affiliated Hospital, Xinjiang Medical University
  • Nakamura Haruhiko
    Department of Respiratory Surgery, Atami Hospital, International University of Health and Welfare
  • Niyazi Maidiniyeti
    Department of Pathology, Tokyo Medical University Emergency Center of Xinjiang People's Hospital
  • Taguchi Masahiko
    Department of Respiratory Surgery, Atami Hospital, International University of Health and Welfare
  • Kawasaki Norihito
    Department of Respiratory Surgery, Atami Hospital, International University of Health and Welfare
  • Ohira Tatsuo
    Department of Surgery, Tokyo Medical University
  • Kato Harubumi
    Department of Surgery, Tokyo Medical University

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  • Ｆｌｕｏｒｅｓｃｅｎｃｅ　ｉｎ　Ｓｉｔｕ　Ｈｙｂｒｉｄｉｚａｔｉｏｎ法による気管支洗浄液中の肺癌細胞検出
  • Fluorescence in Situ Hybridizationホウ ニ ヨル キカンシ センジョウエキ チュウ ノ ハイガン サイボウ ケンシュツ

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Abstract

Although cytological examination plays an important role in definite diagnosis of lung cancer, morphological diagnosis of cancer cells requires specific skills. Thus, a more objective type of diagnostic method is deemed useful. Since numerical abnormalities are frequently seen in cancer cells, detection of chromosomal aberrant numbers in interphase nuclei using fluorescence in situ hybridization (FISH), can be used to identify cancer cells. We compared the results of conventional cytological examination with those of analysis of aberrant copy numbers of chromosomes 3 and 17 in cells in bronchial washings from patients who underwent endoscopic examination to obtain definite diagnosis of lung cancer our hospital. Six cases were positive for lung cancer in both cytology and chromosomal analysis. We found one case of cytology-negative and FISH-positive and one case of cytology-positive and FISH-negative. Two cases were pseudonegative for both examinations. These findings suggest that FISH is useful as an objective marker for detection of malignant cells in lung cancer diagnosis, and can play a complementary role to conventional cytology.

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