RNAi-mediated knock down of mammalian selenocysteine lyase

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Selenocysteine lyase (SCL), which specifically catalyzes the decomposition of L-selenocysteine into Se and L-alanine, is proposed to function as a Se delivery protein to selenophosphate synthetase in selenoprotein biosynthesis. However, the physiological role of SCL has not been established. To address the role of SCL in mammalian cells, we have used RNA-interference (RNAi) to deplete SCL in a HeLa cell line. RNAi-mediated depletion of SCL resulted in growth inhibition in cells cultivated with a serum-containing medium. The similar growth inhibition was observed in SCL-depleted cells cultivated with a serum-free medium supplemented with selenite, selenomethionine, or selenocysteine, indicating that these selenium-containing compounds can not rescue the growth inhibition. These results suggest a physiological importance of SCL in normal cell function. Depletion of SCL may reduce the biosynthesis levels of selenoproteins which are essential for cell proliferation.

収録刊行物

  • Biomedical research on trace elements

    Biomedical research on trace elements 16(4), 321-324, 2005-12-31

    日本微量元素学会

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各種コード

  • NII論文ID(NAID)
    10018624840
  • NII書誌ID(NCID)
    AN10423256
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    0916717X
  • NDL 記事登録ID
    7872425
  • NDL 雑誌分類
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL 請求記号
    Z19-3255
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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