Characterization of Direct Immobilized Probe DNA on Partially Functionalized Diamond Solution-Gate Field-Effect Transistors

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著者

    • Yang Jung-Hoon Yang Jung-Hoon
    • Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
    • Song Kwang-Soup Song Kwang-Soup
    • Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
    • Kuga Shouma [他] Kuga Shouma
    • Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan
    • Kawarada Hiroshi
    • Department of Electronical Engineering and Bioscience, School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan

抄録

Amino groups were functionalized directly on the diamond surface after treating 0.5 monolayer of oxidation for detection of DNAs. Also, immobilization of probe DNAs was carried out directly on the partially aminated diamond without linker molecules. Specific hybridization with 21-mer DNA at a concentration of 100 nM could be clearly detected by two methods, fluorescence microscopy and diamond solution-gate field-effect transistors (SGFETs). DNA hybridization was confirmed using Cy-5-labeled target DNA on a micropatterned diamond surface. The changes in gate potential by the negative charge of immobilized or hybridized DNA were measured on SGFETs and hybridization efficiency on the functionalized diamond surface was estimated as about 40%.

収録刊行物

  • Japanese journal of applied physics. Pt. 2, Letters

    Japanese journal of applied physics. Pt. 2, Letters 45(42), L1114-L1117, 2006-11-25

    Japan Society of Applied Physics

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各種コード

  • NII論文ID(NAID)
    10018632136
  • NII書誌ID(NCID)
    AA10650595
  • 本文言語コード
    EN
  • 資料種別
    SHO
  • ISSN
    0021-4922
  • NDL 記事登録ID
    8548069
  • NDL 雑誌分類
    ZM35(科学技術--物理学)
  • NDL 請求記号
    Z54-J337
  • データ提供元
    CJP書誌  NDL  JSAP 
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