Edwardsiella tarda のHEp-2細胞への付着性に及ぼす培地食塩濃度の影響 Sodium chloride-enhanced adherence of Edwardsiella tarda to HEp-2 cells
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異なる食塩濃度の培地で培養した<i>E. tarda</i> 3株のHEp-2細胞への付着性を比較した。生菌数測定, 直接光顕観察のいずれにおいても, FK1051株およびKG8401株は3%食塩濃度の培地で培養すると0%食塩濃度の培地での培養に比べて高い細胞付着性を示したが, SU166株のそれは培地の塩分濃度にかかわらず低かった。これらの細胞付着性と培地食塩濃度との関係は, 赤血球凝集活性とのそれとよく一致した。また, この細胞付着性はN-アセチルノイラミン酸によって阻害された。
The adherence of <i>Edwardsiella tarda</i> strains to HEp-2 cells was studied by the viable cell count and direct microscopic count methods. Three selected <i>E. tarda</i> strains having different hemagglutination activities against guinea pig erythrocytes were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) or without NaCl (0%-NaCl culture). Strain FK1051 showed significantly higher adherence in the 3%-NaCl culture than in the 0%-NaCl culture. A similar adherence pattern was observed in strain KG8401, though the adherence of the 0%-NaCl culture was higher than that of FK1051. In contrast, both cultures of strain SU166 were low in the adherence. These adherence patterns to the HEp-2 cells correlated well with the previously reported hemagglutinating activities of the strains. <i>N</i>-acetylneuraminic acid markedly inhibited the adherence as well as the hemagglutination. This NaCl-enhanced adherence may be involved in pathogenesis of <i>E. tarda</i> infection particularly in marine fish.
- Fish Pathol.
Fish Pathol. 41(4), 165-170, 2006-12-15
The Japanese Society of Fish Pathology