Sodium chloride-enhanced adherence of Edwardsiella tarda to HEp-2 cells
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- Mahmoud Mahmoud Mostafa
- Graduate School of Biosphere Science, Hiroshima University
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- Okuda Jun
- Graduate School of Biosphere Science, Hiroshima University
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- Nakai Toshihiro
- Graduate School of Biosphere Science, Hiroshima University
Bibliographic Information
- Other Title
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- <i>Edwardsiella tarda</i> のHEp-2細胞への付着性に及ぼす培地食塩濃度の影響
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Abstract
The adherence of Edwardsiella tarda strains to HEp-2 cells was studied by the viable cell count and direct microscopic count methods. Three selected E. tarda strains having different hemagglutination activities against guinea pig erythrocytes were cultured in a peptone-yeast extract broth supplemented with 3% NaCl (3%-NaCl culture) or without NaCl (0%-NaCl culture). Strain FK1051 showed significantly higher adherence in the 3%-NaCl culture than in the 0%-NaCl culture. A similar adherence pattern was observed in strain KG8401, though the adherence of the 0%-NaCl culture was higher than that of FK1051. In contrast, both cultures of strain SU166 were low in the adherence. These adherence patterns to the HEp-2 cells correlated well with the previously reported hemagglutinating activities of the strains. N-acetylneuraminic acid markedly inhibited the adherence as well as the hemagglutination. This NaCl-enhanced adherence may be involved in pathogenesis of E. tarda infection particularly in marine fish.
Journal
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- Fish Pathology
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Fish Pathology 41 (4), 165-170, 2006
The Japanese Society of Fish Pathology
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Keywords
Details 詳細情報について
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- CRID
- 1390282681437729280
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- NII Article ID
- 10018654932
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- NII Book ID
- AN00063165
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- COI
- 1:CAS:528:DC%2BD2sXitlCnsrg%3D
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- ISSN
- 18817335
- 0388788X
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- NDL BIB ID
- 8621380
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed