Mesalazine(Pentasa) の好中球機能に及ぼす影響と Sodium ferrous citrate(SFC) との併用効果についての検討 Evaluation of the combined effect of Mesalazine (Pentasa) and sodium ferrous citrate (SFC) on neutrophil functions

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著者

    • 華 見 HUA Jian
    • 順天堂大学医学部生化学・生体防御学 Department of Host Defense and Biochemical Research, Juntendo University, School of Medicine
    • 秦 政輝 HATA Masaki
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine
    • 前多 力 MAEDA Tsutomu
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine
    • 瀧田 尚仁 TAKITA Naohito
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine
    • 落合 匠 OCHIAI Takumi
    • (財)東京都保健医療公社東部地域病院外科 Department of Surgery, Tobu Chiiki Hospital, Tokyo Metropolitan Health and Medical Treatment Corporation
    • 長岡 功 NAGAOKA Isao
    • 順天堂大学医学部生化学・生体防御学 Department of Host Defense and Biochemical Research, Juntendo University, School of Medicine
    • 鎌野 俊紀 KAMANO Toshiki
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine

抄録

INTRODUCTION: Mesalazine(Pentasa) is widely used for ulcerative colitis(UC). However, the anti-inflammatory actions of Mesalazine are not fully understood. In this study, we evaluated the actions of Mesalazine <I>in vitro</I> on the functions of neutrophil, a major inflammatory cell. Furthermore, we evaluated the combined effects of Mesalazine and sodium ferrous citrate(SFC) on neutrophil functions, since SFC is clinically administered to UC patients together with Mesalazine for treatment of chronic anemia associated with UC.<BR> METHODS: Superoxide production by neutrophils was measured on the basis of superoxide dismutaseinhibitable cytochrome c reduction. Preincubated neutrophils were stimulated with fMLP, PMA or complement-opsonized zymosan particles. Cytochrome c reduction was calculated by the absorbance difference at 550 nm. Neutrophil migration was measured by a Boyden chamber assay with using zymosan-activated serum (C5a) as a chemotactic factor. Neutrophils were preincubated in the presence of 0.01-0.5mM Mesalazine and then incubated with fMLP. The cells were further incubated with phycoerythrin (PE)-labeled anti-CD11b monoclonal antibody, and the expression of CD11b was analyzed by flow cytometry. Neutrophils were preincubated with 5μg/ml SFC and 0.01-0.1mM Mesalazine, and superoxide generation and migration were measured.<BR>RESULTS: Mesalazine dose-dependently inhibited the superoxide production induced by PMA and fMLP at > 0.05 mM. Furthermore, Mesalazine inhibited chemotaxis toward zymosan-activated serum in a dosedependent fashion. Moreover, 0.5mM Mesalazine inhibited the fMLP-induced up-regulation of CD11b expression. Interestingly, Mesalazine combined with SFC inhibited the superoxide generation and migration by neutrophils, compared with Mesalazine alone. Thus, the present observations suggest that a combined administration of Mesalazine and SFC may have a potential to reduce mucosal injury by suppressing neutrophil functions (migration, ROS generation and expression of adhesion molecule) in UC.

収録刊行物

  • 炎症・再生 : 日本炎症・再生医学会雑誌 = Inflammation and regeneration

    炎症・再生 : 日本炎症・再生医学会雑誌 = Inflammation and regeneration 26(1), 49-53, 2006-01-25

    The Japanese Society of Inflammation and Regeneration

参考文献:  14件中 1-14件 を表示

被引用文献:  3件中 1-3件 を表示

各種コード

  • NII論文ID(NAID)
    10018657199
  • NII書誌ID(NCID)
    AA11508953
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    13468022
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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