Sodium ferrous citrate の好中球機能に及ぼす影響 Evaluation of the effect of sodium ferrous citrate on neutrophil functions

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著者

    • 前多 力 MAEDA Tsutomu
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine
    • 華 見 HUA Jian
    • 順天堂大学医学部生化学・生体防御学 Department of Host Defense and Biochemical Research, Juntendo University, School of Medicine
    • 秦 政輝 HATA Masaki
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine
    • 瀧田 尚仁 TAKITA Naohito
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine
    • 落合 匠 OCHIAI Takumi
    • (財)東京都保健医療公社東部地域病院外科 Department of Surgery, Tobu Chiiki Hospital, Tokyo Metropolitan Government Health Care Public Corporation
    • 長岡 功 NAGAOKA Isao
    • 順天堂大学医学部生化学・生体防御学 Department of Host Defense and Biochemical Research, Juntendo University, School of Medicine
    • 鎌野 俊紀 KAMANO Toshiki
    • 順天堂大学医学部下部消化管外科 Department of Coloproctological Surgery, Juntendo University, School of Medicine

抄録

BACKGROUND AND PURPOSE: The inflammatory bowel diseases(IBD) such as ulcerative colitis(UC) are intractable diseases of unknown origin. The mucosal injury of UC is presumed to be partly caused by reactive oxygen species(ROS) produced by neutrophils. Chronic inflammation of UC results in chronic anemia, to which iron is given. In this study, to evaluate the effect of sodium ferrous citrate(SFC) on neutrophil functions, we measured superoxide generation and migration of neutrophils treated with SFC <I>in vitro</I>.<BR>METHODS: Superoxide generation induced by phorbol myristate acetate(PMA), opsonized zymosan(OPZ) and N-formyl-menthionyl-leucyl-phenylalanine(fMLP) was determined based on the superoxide dismutaseinhibitable cytochrome c reduction. To further clarify the inhibitory mechanism of SFC, the membrane fraction was isolated and NADPH-dependent superoxide production was measured. Moreover, neutrophil migration was evaluated with a Boyden chamber assay using zymosan-activated serum (C5a) as a chemoattractant.<BR>RESULTS: SFC dose-dependently inhibited the superoxide production induced by all the stimuli examined. Similarly, SFC-treatment inhibited the activity of NADPH oxidase in the membrane fraction of neutrophils. In addition, SFC suppressed chemotaxis toward zymosan-activated serum in a dose-dependent fashion.<BR>CONCLUSIONS: In this study, we revealed that SFC could suppress neutrophil functions such as superoxide generation (NADPH oxidase activity) and migration. During episodes of IBD, stimulated neutrophils are accumulated in inflamed mucosa via the migration through vascular endothelium, where they release ROS such as superoxide. Thus, the present observations suggest that SFC may have a potential to reduce mucosal injury by suppressing migration and ROS generation by neutrophils in UC.

収録刊行物

  • 炎症・再生 : 日本炎症・再生医学会雑誌 = Inflammation and regeneration  

    炎症・再生 : 日本炎症・再生医学会雑誌 = Inflammation and regeneration 26(1), 54-58, 2006-01-25 

    The Japanese Society of Inflammation and Regeneration

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各種コード

  • NII論文ID(NAID)
    10018657214
  • NII書誌ID(NCID)
    AA11508953
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    13468022
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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