Developmental Stages and Germ Cell Lineage of the Loach (Misgurnus anguillicaudatus)

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著者

    • Kataoka Takashi KATAOKA Takashi
    • Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University
    • Sakao Suzu [他] SAKAO Suzu
    • Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University
    • SAITO Taiju
    • Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University
    • YAMAHA Etsuro
    • Nanae Fresh Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University
    • ARAI Katsutoshi
    • Laboratory of Aquaculture Genetics and Genomics, Division of Marine Life Science, Graduate School of Fisheries Sciences, Hokkaido University

抄録

The staging of embryonic and larval development, and the germ cell lineage of the loach, Misgurnusanguillicaudatus, are described. Fertilized eggs were obtained by artificial insemination. For theconvenience of detailed observation and photography of the external appearance, we use dechorionatedembryos. Through a series of operations, these embryos were cultured at 20°C in an incubator.Embryonic and larval development of the loach was divided into five periods: cleavage,blastula, gastrula, segmentation, and hatching. Stages were assigned within each of these periods.Developmental stages were determined and named by morphological features and somite number.The staging series were photographed and tabulated. The germ cell lineage was then elucidatedby whole mount in situ hybridization of mRNA expression of the germ-cell-specific marker vasa andhistological analysis. Primordial germ cells (PGCs) of the loach derived from the cleavage furrowsof 8-cell stage embryos began proliferation in the late blastula period and migrated to the gonadalanlagen through a migration pathway similar to that of the zebrafish. However, it is characteristicof the loach that PGCs migrate a long distance and stay in the posterior part of the yolk-extensionregion.

The staging of embryonic and larval development, and the germ cell lineage of the loach, Misgurnus anguillicaudatus, are described. Fertilized eggs were obtained by artificial insemination. For the convenience of detailed observation and photography of the external appearance, we use dechorionated embryos. Through a series of operations, these embryos were cultured at 20°C in an incubator. Embryonic and larval development of the loach was divided into five periods: cleavage, blastula, gastrula, segmentation, and hatching. Stages were assigned within each of these periods. Developmental stages were determined and named by morphological features and somite number. The staging series were photographed and tabulated. The germ cell lineage was then elucidated by whole mount in situ hybridization of mRNA expression of the germ-cell-specific marker vasa and histological analysis. Primordial germ cells (PGCs) of the loach derived from the cleavage furrows of 8-cell stage embryos began proliferation in the late blastula period and migrated to the gonadal anlagen through a migration pathway similar to that of the zebrafish. However, it is characteristic of the loach that PGCs migrate a long distance and stay in the posterior part of the yolk-extension region.

収録刊行物

  • Zoological science  

    Zoological science 23(11), 977-989, 2006-11-25 

    日本動物学会

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各種コード

  • NII論文ID(NAID)
    10018662056
  • NII書誌ID(NCID)
    AA10545874
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    02890003
  • NDL 記事登録ID
    8587034
  • NDL 雑誌分類
    ZR4(科学技術--生物学--動物)
  • NDL 請求記号
    Z53-Y164
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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