N-Terminus Methylation of Desulfovibrio vulgaris (Miyazaki) F Cytochrome c_3
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Cytochrome <I>c</I><SUB>3</SUB> from <I>Desulfovibrio vulgaris</I> (Miyazaki) F is an electron-transfer protein between hydrogenase and ferredoxin and other types of cytochromes. In this study, an N-terminus of the cytochrome <I>c</I><SUB>3</SUB> has been identified as an N-methylated alanine residue by cleavage with peptidase, liquid chromatography/mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR) analysis. Heterologous expression of the cytochrome <I>c</I><SUB>3</SUB> with <I>Shewanella oneidensis</I> TSP-C is not methylated, this indicate that the N-methylation occur in vivo in <I>D. vulgaris</I>.
- Chemistry Letters
Chemistry Letters 36(1), 184-185, 2007-01-05
The Chemical Society of Japan