Dibutyltin (DBT) Dichloride Inhibits Cytokine Productions in Murine Macrophage Cell Line, J774.1

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著者

    • INOUE Yoko
    • Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
    • MIKI Takeo
    • Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
    • KUDO Yuichiro
    • Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
    • SATOH Toshihiko
    • Department of Preventive Medicine and Public Health, Kitasato University School of Medicine
    • AIZAWA Yoshiharu
    • Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

抄録

The immune system is a target of dibutyltin (DBT) intoxication. We evaluated the effects of DBT dichloride in macrophages using the murine macrophage cell line, J774.1. Cultured J774.1 cells were exposed to DBT dichloride at 0, 0.5, 1.0, 1.5 or 2.0 <I>μ</I>M in 24-well plates. After 18 hours, lipopolysaccharide was added to each well. The cells were incubated for an additional 6 hours or 24 hours. At the ends of the incubations, the cell viability was determined by the trypan blue exclusion method. Total RNA was extracted from the cells after an additional 6 hours of incubation. Real- time polymerase chain reaction (PCR) was used to analyze the mRNA expression for tumor necrosis factor <I>α</I> (TNF<I>α</I>), interleukin 1<I>β</I> (IL-1<I>β</I>) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (housekeeping gene) in J774.1 cells. The supernatants were sampled after an additional 24 hours of incubation. The concentrations of TNF<I>α</I> and IL-1<I>β</I> in the supernatants were determined by ELISA. The mean values of cell viabilities in the DBT-exposed groups were significantly lower than that of respective control both after the additional 6 and 24 hours of incubation. The mean relative mRNA expression of TNF<I>α</I> was higher than that in the control only in the 0.5 <I>μ</I>M group. There were no significant differences in IL-1<I>β</I> relative mRNA expression among the groups. The mean concentrations of TNF<I>α</I> in the supernatant in the 1.0, 1.5 and 2.0 <I>μ</I>M groups were significantly lower compared to that in the control. The mean concentrations of IL-1<I>β</I> in the supernatant in the DBT-exposed groups were also significantly lower than that of the control. The concentrations of cytokines in the supernatants were marked low and not fully explained by the low cell viability of the DBT-exposed groups. Since the mRNA expressions of cytokines in the higher dose groups were similar to that in the control, the translation from mRNA may be inhibited by DBT.

収録刊行物

  • Biomedical research on trace elements  

    Biomedical research on trace elements 17(4), 417-422, 2006-12-31 

    Japan Society for Biomedical Research on Trace Elements

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各種コード

  • NII論文ID(NAID)
    10018664594
  • NII書誌ID(NCID)
    AN10423256
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    0916717X
  • NDL 記事登録ID
    8664970
  • NDL 雑誌分類
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL 請求記号
    Z19-3255
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
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