Influence of Preservation in Two Kinds of Formaldehyde Solutions on the Fracture Characteristics of Bovine Femoral Compact Bones

• Asaka Takashi

  Department of Applied Chemistry, School of Engineering, Tokai University

• Kikugawa Hisao

  Department of Network and Computer Engineering, School of Information and Design Engineering, Tokai University

抄録

For the evaluation of the physical properties of bones, specimens are often either deep-frozen or chemically fixed with reagents such as formaldehyde (formalin) or ethanol for antisepsis and sterilization. However, formalin contains formic acid, which dissolves bone minerals such as Ca and P. To suppress this bone mineral elution, we tested neutral buffered formalin as a fixative. In this study, we investigated the influence of formalin and the neutral buffered formalin fixation on the fracture characteristics of bovine femoral compact bones over a relatively long-term preservation period. In both the formalin and the neutral buffered formalin fixation, bone minerals migrated rapidly into the fixatives. Thus, some aqueous solutions such as a normal saline solution may also dissolve bone minerals. In the neutral buffered formalin fixation, fine calcium phosphate grains precipitated on the surface of the soft tissues of blood vessels in the Haversian canal, as observed by scanning electron microscopy. An element analysis with energy-dispersive X-ray spectroscopy also demonstrated the presence of Ca and P. Thus, the precipitated grains are assumed to be hydroxyapatite. In this study we evaluated the influence of formalin preservation, which reduces the fracture toughness of bovine femoral compact bones, and concluded that due to the formation of chemical bridges from the reaction of formaldehyde with the collagen fibers in the bones, the collagen fibers are cured and hardened, resulting in a reduction in the fracture toughness of the bovine femoral compact bones.

収録刊行物

• MATERIALS TRANSACTIONS

  MATERIALS TRANSACTIONS 48 (1), 16-20, 2007

  公益社団法人 日本金属学会

キーワード

• bone
• mechanical properties
• fracture toughness
• mechanical test
• formalin preservation
• mineral elution
• quantitative element analysis

詳細情報

• CRID: 1390282679226159872
• NII論文ID: 130004453331
• NII論文ID: 10018665970
• NII書誌ID: AA1151294X
• DOI: 10.2320/matertrans.48.16
• ISSN: 13475320
• ISSN: 13459678
• NDL書誌ID: 8597174
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8597174
• Web Site: https://www.jstage.jst.go.jp/article/matertrans/48/1/48_1_16/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可