Transplantation of adult neural stem cells modified to secrete GDNF has neuroprotective and restorative effect in ischemia model of rats
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- Kameda Masahiro
- Department of Neurological Surgery, Okayama Univ. Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
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- Shingo Tetsuro
- Department of Neurological Surgery, Okayama Univ. Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
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- Uozumi Takashi
- Department of Neurological Surgery, Okayama Univ. Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
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- Matsui Toshihiro
- Department of Neurological Surgery, Okayama Univ. Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
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- Miyoshi Yasuyuki
- Department of Neurological Surgery, Okayama Univ. Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
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- Date Isao
- Department of Neurological Surgery, Okayama Univ. Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences
Bibliographic Information
- Other Title
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- 虚血モデルに対する成体由来神経幹細胞を用いた神経再生・保護効果の検討
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Abstract
The aim of cell transplantation is to replace damaged area. But transplanted neural stem/progenitor cells (NSPCs) didn't treat lesion core. We transplanted adult NSPCs modified to secrete GDNF in order to make transplanted NSPCs replace ischemic area more effectively.<BR>NSPCs were harvested from subventricular zone (SVZ) of adult rats and cultured with EGF by using neurosphere technique. Expanded NSPCs were transfected with fiber-mutant F/RGD adenovirus containing GDNF (NSPC-GDNF) or EGFP (NSPC-EGFP) gene. The best transfection efficiency was derived from GDNF ELISA. At first, NSPC-GDNF or NSPC-EGFP cells were transplanted into the ischemic boundary zone of MCAO model of Wistar rats in the acute stage (allogenic transplantation). NSPC-GDNF group had a significantly better result in behavioral test and infarction volume than NSPC-EGFP group. Subsequently, NSPC-GDNF or NSPC-EGFP cells were transplanted into the damaged CAI of global ischemia model of Fischer344 rats in the chronic stage (syngenic transplantation). NSPC-GDNF cells migrated and differentiated into neuron as replacing the damaged CAI of hippocampus partially. Moreover, NSPC-GDNF group had shown the activation of neurogenesis of endogenous NSPCs.<BR>Consequently, we confirm NSPC-GDNF have neuroprotective effect and can replace damaged area. We think this result suggests that NSPC-GDNF cells can bring a good result in autologous-transplantation.
Journal
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- Japanese Journal of Stroke
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Japanese Journal of Stroke 28 (4), 613-617, 2006
The Japan Stroke Society
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Details 詳細情報について
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- CRID
- 1390001204639927424
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- NII Article ID
- 10018686850
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- NII Book ID
- AN0020186X
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- ISSN
- 18831923
- 09120726
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- Text Lang
- ja
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed