Expression of the osteoblastic marker in human alveolar bone cells spheroid

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  • ヒト歯槽骨由来細胞スフェロイドにおける骨芽細胞マーカーの発現

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Abstract

We previously reported the presence of type I, and III collagen (Col-I, III) and proliferating cells in human alveolar bone cells (HABCs) three-dimensional (3D) grafts (spheroid). The existence and differentiation of osteoblasts in the HABC spheroid remains to be clarified, and the function of the HABCs spheroid is poorly understood. We studied the HABCs spheroid on different cultivation days in histological and immunohistochemical staining, using the monoclonal antibody of the expression of Ki-67, Col-I, osteopontin (OPN), and osteocalcin (OC). HABCs differentiation was assessed by alkaline phosphatase (ALP) staining. The number of Ki-67 antigen-positive nuclei was observed in days 1, 3, 7, and 21. During the 21-day study,the positive Col-I reaction of increased and the positive ALP reaction decreased time-dependently. Positive staining with OPN and OC were observed only at day 21 after spheroid formation. These results indicate the entity and differentiation of the osteoblast in the HABCs spheroid and the possibility of mineralization in vitro. Nihon Shishubyo Gakkai Kaishi (J Jpn Soc Periodontol) 48 : 276-284, 2006.

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