Evaluation of the Recognition Ability of Molecularly Imprinted Proteins by Surface Plasmon Resonance (SPR) Spectroscopy

• Yoshikawa Masakazu

  Laboratory for Applied Polymer Chemistry, Department of Biomolecular Engineering, Kyoto Institute of Technology

• Kawamura Kensuke

  Laboratory for Applied Polymer Chemistry, Department of Biomolecular Engineering, Kyoto Institute of Technology

• Watanabe Kunihiko

  Laboratory of Microbial Physiology and Applied Microbiology, Department of Applied Biochemistry, Kyoto Prefectural University

Abstract

Cell membrane proteins from Geobacillus thermodenitrificans DSM465 were converted into the adenosine (As)　recognition materials by adopting 9-ethyladenine (9-EA) as a print molecule. The converted molecular recognition　sites gave the affinity constant of 4.62 × 104 mol–1 dm3 toward As, which was determined by SPR spectroscopy. It　was demonstrated that cell membrane proteins from G. thermodenitrificans DSM465 would be one of candidate materials　to be converted into molecular recognition materials via an alternative molecular imprinting.

Journal

• MEMBRANE

  MEMBRANE 32 (1), 40-44, 2007

  THE MEMBRANE SOCIETY OF JAPAN

Keywords

• adenosine
• adsorption
• 9-ethyladenine
• Geobacillus thermodenitrificans DSM465
• green polymer
• guanosine
• molecular imprinting
• molecular recognition
• protein

Details

• CRID: 1390001206421669888
• NII Article ID: 10018696330
• NII Book ID: AN0023215X
• DOI: 10.5360/membrane.32.40
• ISSN: 18846440
• ISSN: 03851036
• NDL BIB ID: 8662512
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I8662512
• Web Site: https://www.jstage.jst.go.jp/article/membrane/32/1/32_40/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed