透明導電性フィルムを用いた培養細胞の直接質量分析法の開発 Direct Analysis of Cultured Cells with Matrix-Assisted Laser Desorption/Ionization on Conductive Transparent Film

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著者

    • 杉浦 悠毅 SUGIURA Yuki
    • 東京工業大学大学院生命理工学研究科 Department of Bioscience and Biotechnology, Tokyo Institute of Technology
    • 新間 秀一 SHIMMA Shuichi
    • 自然科学研究機構岡崎統合バイオサイエンスセンター Okazaki Institute for Integrative Bioscience, National Institutes of Natural Sciences

抄録

Direct tissue analysis and ion imaging using mass spectrometry extract significant information directly from complex biological samples. In this paper, we introduce a method for the direct analysis of cultured cells. To analyze cultured cells by direct mass spectrometry (MS), we incubated HEK293T cells directly on an indium-tin-oxide layer on polyethylene terephthalate film. After fixation of cells on the film, matrix application was performed by spray-droplet method, which is a two-step matrix application method to improve the ionization efficiency of biological tissue samples. By direct MS of HEK293T cells, we obtained mass spectra of approximately 350 protein signals with good quality. Furthermore, we profiled proteins as digested products and identified major signals by applying an on-tissue digestion direct MS/MS method, which is the procedure for enzymatic protein digestion of biological tissue samples. This new approach enables proteomic analysis directly from the cell culture system.

収録刊行物

  • 質量分析 = Mass spectroscopy  

    質量分析 = Mass spectroscopy 55(1), 25-31, 2007-02-01 

    The Mass Spectrometry Society of Japan

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各種コード

  • NII論文ID(NAID)
    10018702980
  • NII書誌ID(NCID)
    AN0010555X
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    13408097
  • NDL 記事登録ID
    8675260
  • NDL 雑誌分類
    ZP4(科学技術--化学・化学工業--分析化学)
  • NDL 請求記号
    Z17-213
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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