Development of a Diagnostic Shuttle PCR Test Targeting the Streptococcus equi SeM Gene
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- ANZAI Toru
- Epizootic Research Center, Equine Research Institute, Japan Racing Association
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- HOBO Seiji
- Epizootic Research Center, Equine Research Institute, Japan Racing Association
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- NIWA Hidekazu
- Epizootic Research Center, Equine Research Institute, Japan Racing Association
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A shuttle PCR test targeting the conserved region of the SeM gene of Streptococcus equi was developed as a method for detecting this bacterium from horses. An amplicon 289 bp in size was detected from 137 strains of S. equi, but was not seen in 517 strains of other bacteria, including S. zooepidemicus and in 227 nasal mucosal swabs taken from horses which S. equi had not been isolated. A comparative examination using 12 strains of S. equi in serial dilution and 73 swabs taken from the nasal mucosa of horses from which S. equi had been isolated showed the sensitivity of our PCR was slightly inferior to conventional PCR described in a previous report, even though it was enough to use as a diagnostic tool. In conclusion, we suggest that the PCR method described here has advantages with respect to specificity and sensitivity as a diagnostic test for strangles.<br>
収録刊行物
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- Journal of Equine Science
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Journal of Equine Science 17 (4), 101-104, 2006
日本ウマ科学会
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詳細情報 詳細情報について
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- CRID
- 1390001204368707456
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- NII論文ID
- 10018710108
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- NII書誌ID
- AA11010806
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- ISSN
- 13477501
- 13403516
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- NDL書誌ID
- 8654605
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- CiNii Articles
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