Identification of Horse Anti-Ferritin Autoantibody

この論文にアクセスする

この論文をさがす

著者

抄録

Ferritin-binding proteins (FBPs) in horse serum were characterized by immunoblotting and ferritin-binding experiments. FBPs purified from horse serum by horse spleen ferritin-Sepharose 4B affinity chromatography were separated into two fractions by Sephacryl S-300 gel chromatography: FBP 1 and FBP 2. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, FBP 1 separated into 79.0- and 25.3-kDa bands, and FBP 2 separated into 62.7- and 25.3-kDa bands. Immunoblotting analysis using antibodies specific for horse IgM and IgA heavy chains and IgG F(c) fragment showed that the 79.0- and 62.7-kDa bands were IgM and IgG heavy chains, respectively. After forming complexes of horse FBPs with horse spleen ferritin, rabbit anti-horse ferritin antibody was used to form immune complexes against ferritin, allowing co-precipitation and subsequent identification by monoclonal antibodies to horse immunoglobulin (IgM, IgA, IgGa and IgGb). Horse serum was positive for IgM, IgGa, IgGb and IgA complexes and affinity-purified FBPs for IgM and IgGb complexes. FBP 1 was identified as an IgM complex that forms with ferritin but FBP 2 did not form any complexes with ferritin. These results suggest that circulating horse serum ferritin is in the form of IgM, IgG (IgGa and IgGb) and IgA complexes.<br>

収録刊行物

  • Journal of equine science = Continuation of Japanese Journal of Equine Science and Bulletin of Equine Research Institute  

    Journal of equine science = Continuation of Japanese Journal of Equine Science and Bulletin of Equine Research Institute 17(4), 113-119, 2006-12-01 

    Japanese Society of Equine Science

参考文献:  31件

参考文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

被引用文献:  2件

被引用文献を見るにはログインが必要です。ユーザIDをお持ちでない方は新規登録してください。

各種コード

  • NII論文ID(NAID)
    10018710145
  • NII書誌ID(NCID)
    AA11010806
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13403516
  • NDL 記事登録ID
    8654623
  • NDL 雑誌分類
    ZR4(科学技術--生物学--動物)
  • NDL 請求記号
    Z18-3142
  • データ提供元
    CJP書誌  CJP引用  NDL  J-STAGE 
ページトップへ