-
- ORINO Koichi
- Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University
-
- HAMADA Shuichiro
- Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University
-
- HASHIMOTO Masafumi
- Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University
-
- NAMBO Yasuo
- Equine Science Division, Hidaka Training Research Center, Japan Racing Association
-
- KONDO Takashi
- Epizootic Research Station, Equine Research Institute, Japan Racing Association
-
- WATANABE Kiyotaka
- Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University
この論文をさがす
抄録
Ferritin-binding proteins (FBPs) in horse serum were characterized by immunoblotting and ferritin-binding experiments. FBPs purified from horse serum by horse spleen ferritin-Sepharose 4B affinity chromatography were separated into two fractions by Sephacryl S-300 gel chromatography: FBP 1 and FBP 2. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing conditions, FBP 1 separated into 79.0- and 25.3-kDa bands, and FBP 2 separated into 62.7- and 25.3-kDa bands. Immunoblotting analysis using antibodies specific for horse IgM and IgA heavy chains and IgG F(c) fragment showed that the 79.0- and 62.7-kDa bands were IgM and IgG heavy chains, respectively. After forming complexes of horse FBPs with horse spleen ferritin, rabbit anti-horse ferritin antibody was used to form immune complexes against ferritin, allowing co-precipitation and subsequent identification by monoclonal antibodies to horse immunoglobulin (IgM, IgA, IgGa and IgGb). Horse serum was positive for IgM, IgGa, IgGb and IgA complexes and affinity-purified FBPs for IgM and IgGb complexes. FBP 1 was identified as an IgM complex that forms with ferritin but FBP 2 did not form any complexes with ferritin. These results suggest that circulating horse serum ferritin is in the form of IgM, IgG (IgGa and IgGb) and IgA complexes.<br>
収録刊行物
-
- Journal of Equine Science
-
Journal of Equine Science 17 (4), 113-119, 2006
日本ウマ科学会
- Tweet
詳細情報
-
- CRID
- 1390282679345404800
-
- NII論文ID
- 10018710145
-
- NII書誌ID
- AA11010806
-
- ISSN
- 13477501
- 13403516
-
- NDL書誌ID
- 8654623
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可