Gene-centromere Mapping at Four Microsatellite and Five Isozyme Loci in Clarias macrocephalus Based on Meiotic Gynogenetic Offspring

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  • 極体放出阻止型雌性発生二倍体を用いた<I>Clarias macrocephalus</I>におけるマイクロサテライト4座およびアイソザイム5座のG-Cマッピング

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Abstract

Gene-centromere distance was estimated for 4 microsatellite loci, Cma-2, Cma-3, Cma-4 and Cga-3, and 5 isozyme loci, sMDH-1, PGM, GPI-1, ADH, and EST, based on gynogenetic families of Clarias macrocephalus. Differences of genotypic ratio between families were observed at Cma-3, sMDH-1 and GPI-1. We observed different frequencies between the two homozygous classes at sMDH-1 and GPI-1. The recombination rates (y) of three loci exceeded the maximum 67%, thus support the evidence for complete interference along chromosomes of C. macrocephalus. The gene-centromere map distances ranged from 0.6 cM for the Cga-3 to 49 cM for EST. Three loci, Cma-4, Cga-3 and GPI-1, were closely linked to their centromeres. We observed linkage between sMDH-1 and Cma-3 in one out of the three gynogenetic families. Fixation index, estimated by (1-y), was 0.570.

Journal

  • Aquaculture Science

    Aquaculture Science 54 (4), 505-513, 2006

    Japanese Society for Aquaculture Science

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