FISH analysis of the W chromosome in Bombyx mandarina and several other species of Lepidoptera by means of B. mori W-BAC probes
-
- Yoshido Atsuo
- Laboratory of Applied Molecular Entomology, Graduate School of Agriculture, Hokkaido University
-
- Yasukochi Yuji
- National Institute of Agrobiological Sciences, Tsukuba, Japan
-
- Marec František
- Biological Centre, ASCR, Institute of Entomology and Faculty of Biological Sciences University of South Bohemia
-
- Abe Hiroaki
- Department of Biological Production, Tokyo University of Agriculture and Technology
-
- Sahara Ken
- Laboratory of Applied Molecular Entomology, Graduate School of Agriculture, Hokkaido University
書誌事項
- タイトル別名
-
- FISH Analysis of the W Chromosome in <i>Bombyx mandarina</i> and Several Other Species of Lepidoptera by Means of <i>B. mori</i> W-BAC Probes
この論文をさがす
抄録
We newly isolated seven W chromosome derived bacterial artificial chromosome (W-BAC) clones from Bombyx mori BAC libraries by means of six different STS primer sets, which amplify each different female specific DNA fragment. Two color fluorescence in situ hybridization (FISH) with 19L6H W-BAC probe as a standard W-chromosome painter disclosed that six out of seven new W-BAC probes strongly highlighted the whole W chromosome in B. mori. Besides the W, hybridization signals occurred in telomeric and/or subtelomeric regions of the autosomes. The remaining W-BAC, 20H9D, provided discontinuous signals, which appeared in both terminal and central compartments of the W chromosome. A representative of W-chromosome painting BACs, 19L6H, and 20H9D W-BAC were hybridized to chromosome spreads of Bombyx mandarina females. The 19L6H W-BAC bound to the whole B. mandarina W chromosome as in B. mori. In contrast, 20H9D W-BAC signals in B. mandarina W chromosome were different from those in B. mori W, indicating rearrangements of the W chromosome during karyotype evolution of the two closely related Bombyx species. Further FISH analyses using B. mori W-BAC probes in 15 lepidopteran species showed no hybridization signals in their sex chromosomes as well as autosomes. Our results suggest the absence of homologous sequences between the Bombyx W and the W chromosomes of other lepidopteran species.<br>
収録刊行物
-
- Journal of Insect Biotechnology and Sericology
-
Journal of Insect Biotechnology and Sericology 76 (1), 1_1-1_7, 2007
社団法人 日本蚕糸学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390282680173893376
-
- NII論文ID
- 130004464125
- 10018742816
-
- NII書誌ID
- AA11558849
-
- ISSN
- 18847978
- 13468073
-
- NDL書誌ID
- 8667916
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可