Sericin digestion by fibroinase, a cathepsin L-like cysteine proteinase, of Bombyx mori silk gland
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- Watanabe Masahide
- Division of Biology, Kyoto Institute of Technology
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- Kamei Kaeko
- Division of Biology, Kyoto Institute of Technology
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- Sumida Motoyuki
- Division of Biology, Kyoto Institute of Technology
書誌事項
- タイトル別名
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- Sericin Digestion by Fibroinase, a Cathepsin L-like Cysteine Proteinase, of <i>Bombyx mori</i> Silk Gland
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In order to obtain conclusive evidence that fibroinase, a cathepsin L-like cysteine proteinase, of silk gland digests sericin, in addition to fibroin, three sericin fractions, s-A, s-M and s-P, were prepared from cocoons by the method of Takasu et al. (2002) and subjected to enzymatic digestion by fibroinase purified from silk gland of day one B. mori pupa. Time course study showed clearly that all the sericin fractions were digested in a time dependent manner and that complete digestion was attained by 3h. Sericin fragments from each sericin fraction digested for 3h were subjected to reverse phase column chromatography. Mass of each sericin fragment was determined by MALDI TOF mass spectrometry to check the purity of each peak fraction, followed by peptide sequencing by protein sequencer. Some sequences of sericin fragments corresponded portions of the sequence of sericin protein deduced from the nucleotide sequence of sericin gene (Garel et al., 1997). By alignment of an amino acid sequence of a sericin fragment with the sericin protein sequence, estimation of cleavage sites of sericin by fibrionase of silk gland was possible. It was concluded that fibroinase of silk gland digests sericin as well as fibroin (Watanabe et al., 2006a). This is because fibroinase of silk gland is a cathepsin L-like cysteine proteinase, which is generally believed to show broad substrate specificity. Fibroinase of silk gland is highly likely the proteinase that is responsible for digestion of fibroin and sericin in the luminal contents of silk gland in the early pupa and at each molt period during larval development.<br>
収録刊行物
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- Journal of Insect Biotechnology and Sericology
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Journal of Insect Biotechnology and Sericology 76 (1), 1_9-1_15, 2007
社団法人 日本蚕糸学会
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詳細情報 詳細情報について
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- CRID
- 1390001205197170176
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- NII論文ID
- 130004464132
- 10018742852
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- NII書誌ID
- AA11558849
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- ISSN
- 18847978
- 13468073
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- NDL書誌ID
- 8667922
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可