The principal of 2-D fluorescence difference gel electrophoresis

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  • 蛍光ディファレンスゲル二次元電気泳動の原理-蛍光標識二次元ディファレンス電気泳動: 2-D Fluorescence Difference Gel Electrophoresis: Ettan<sup>TM</sup> DIGEシステム
  • ケイコウ ディファレンス ゲル 2ジゲン デンキ エイドウ ノ ゲンリ ケイコウ ヒョウシキ 2ジゲン ディファレンス デンキ エイドウ 2 D Fluorescence Difference Gel Electrophoresis Ettan DIGE システム

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Abstract

Fluorescence 2-D difference gel electrophoresis (DIGE) uses spectrally resolvable dyes to label protein samples prior to 2-D electrophoresis. By using different fluorescent dyes to separately label protein samples multiple samples can be co-separated and visualized on a single 2-D gel. Differences between samples are resolved using image analysis software such as DeCyder 2D. This fluorescent multiplexing approach is compatible with mass spectrometry and overcomes many of the disadvantages of traditional 2-D analyses. A broad dynamic range provides more accurate quantitative data than traditional 2-D silver staining techniques while rapid image overlay simplifies image analysis and improves comparative accuracy.

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