Correlation of Abnormal Mitochondrial Distribution in Mouse Oocytes with Reduced Developmental Competence

• Nagai Seiichiro

  Department of Obstetrics and Gynecology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• Mabuchi Tadashi

  Department of Biochemistry, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• Hirata Shuji

  Department of Obstetrics and Gynecology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• Shoda Tomoko

  Department of Obstetrics and Gynecology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• Kasai Tsuyoshi

  Department of Obstetrics and Gynecology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• Yokota Sadaki

  Biology Laboratory, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

• Shitara Hiroshi

  Department of Laboratory Animal Science, Tokyo Metropolitan Institute of Medical Science

• Yonekawa Hiromichi

  Department of Laboratory Animal Science, Tokyo Metropolitan Institute of Medical Science

• Hoshi Kazuhiko

  Department of Obstetrics and Gynecology, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi

抄録

Selection of good quality oocytes is important for improvement of assisted reproductive technology. Here, we studied the relationship of the mitochondrial distribution in metaphase II stage (MII) oocytes with fertility, since mitochondria in ooplasm are essential for energy production required for fertilization and embryo development. To observe mitochondria non-invasively, we used oocytes from a transgenic mouse, in which enhanced green fluorescent protein is targeted to the mitochondrial matrix and thus fluorescence is observed exclusively in the mitochondria. Control oocytes with mitochondria distributed around the nucleus showed normal embryo developmental competence, whereas oocytes with abnormal diffuse and fragmented mitochondria showed a significantly lower rate of embryo development after activation by intracytoplasmic sperm injection or strontium, which is a very effective agent for activation of mouse oocytes. Also, we showed that the reduced developmental competence of oocytes with diffuse and fragmented mitochondria caused by vitrification and thawing is similar to that of oocytes with abnormal mitochondrial foci obtained naturally. These findings suggest that abnormal mitochondrial distribution in oocytes at MII is a cause of developmental retardation and therefore normal mitochondrial distribution could be used as a criterion for selection of good oocytes.

収録刊行物

• The Tohoku Journal of Experimental Medicine

  The Tohoku Journal of Experimental Medicine 210 (2), 137-144, 2006

  東北ジャーナル刊行会

キーワード

• embryonic development
• mitochondria
• mitochondrial distribution
• mtGFP
• fertilization

詳細情報

• CRID: 1390001204240645376
• NII論文ID: 130004459517
• NII論文ID: 10018771325
• NII書誌ID: AA00863920
• DOI: 10.1620/tjem.210.137
• ISSN: 13493329
• ISSN: 00408727
• Web Site: http://www.jstage.jst.go.jp/article/tjem/210/2/210_2_137/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2007062104
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可