4カラーデジタルフローサイトメーターを用いた小児白血病マーカー中央診断の試み A trial of central diagnosis of childhood acute lymphoblastic leukemia with 4-color digital flow cytometer

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著者

    • 塩沢 裕介 SHIOZAWA Yusuke
    • 国立成育医療センター研究所 発生・分化研究部 Department of Developmental Biology, National Research Institute for Child Health and Development
    • 北村 紀子 KITAMURA Noriko
    • 国立成育医療センター研究所 発生・分化研究部 Department of Developmental Biology, National Research Institute for Child Health and Development
    • 竹野内 寿美 TAKENOUCHI Hisami
    • 国立成育医療センター研究所 発生・分化研究部 Department of Developmental Biology, National Research Institute for Child Health and Development
    • 田口 智子 TAGUCHI Tomoko
    • 国立成育医療センター研究所 発生・分化研究部 Department of Developmental Biology, National Research Institute for Child Health and Development
    • 大喜多 肇 OKITA Hajime
    • 国立成育医療センター研究所 発生・分化研究部 Department of Developmental Biology, National Research Institute for Child Health and Development
    • 小原 明 OHARA Akira
    • 東邦大学医学部大森病院 輸血部 Department of Blood Transfusion, Toho University Omori Medical Center
    • 花田 良二 HANADA Ryoji
    • 埼玉県立小児医療センター 血液・腫瘍科 Department of Hematology and Oncology, Saitama Children's Medical Center
    • 藤本 純一郎 FUJIMOTO Junichiro
    • 国立成育医療センター研究所 発生・分化研究部 Department of Developmental Biology, National Research Institute for Child Health and Development
    • 清河 信敬 KIYOKAWA Nobutaka
    • 国立成育医療センター研究所 発生・分化研究部 Department of Developmental Biology, National Research Institute for Child Health and Development

抄録

<p>Aim. We are in charge of the central diagnosis and cell preservation as a part of childhood acute lymphoblastic leukemia treatment study in Tokyo Children's Cancer Study Group. It is necessary to diagnose with a minimal quantity of specimen, to preserve leukemic cells effectively as possible. Therefore a diagnosis of childhood acute lymphoblastic leukemia by four-color analysis with digital flow cytometer has been examined.</p><p>Methods. We examined cell markers of childhood acute lymphoblastic leukemia cells by four-color analysis using digital flow cytometers. We selected the monoclonal antibodies for the diagnosis based on the recommendation of Japan Pediatric Lymphoma Study Group and made out a panel of antibodies which enable us to confirm aberrant antigen-expressions on the leukemic cells.</p><p>Results. Four colors that we used in this study were fluorescein isothyocyanate, phycoerythrin, phycoerythrin-cyanin 5.1, and phycoerythrin-cyanin-7. The most of childhood acute lymphoblastic leukemia cases could be diagnosed without CD45-gating. List mode compensation was useful to re-investigate specimens which was difficult to re-examine, because there were very few.</p><p></p><p>Discussion. Four-color analysis using digital flow cytometer is useful to save precious specimen of childhood acute lymphoblastic leukemia. We are intending to perform five-color analysis with CD45-gating as a next step.</p>

収録刊行物

  • Cytometry research = 日本サイトメトリー学会機関誌

    Cytometry research = 日本サイトメトリー学会機関誌 16(2), 11-17, 2006-09-25

    日本サイトメトリー学会

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