フローサイトメトリーを用いた細胞周期解析II : BrdU連続ラベル法と細胞周期時間 Cell Cycle Analysis using Flow Cytometry II : BrdU Continuous Labeling and Cell Cycle Time

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著者

    • 宮越 稔 MIYAGOSHI Minoru
    • 金沢医科大学総合医学研究所細胞医学研究部門 Division of Cell Medicine, Research Institute of Medical Science, Kanazawa Medical University

抄録

<p>The BrdU continuous labeling method was presented for a method that can determine the cell cycle time. In this method, the relationships are derived using an equation that n( t,τ) =αN<sub>0</sub>exp(αt) exp (ατ), where n (t,τ) is cell density of a phase τ at time t, N<sub>0</sub> is the initial cell number at time t = 0, Tc is cell-cycle time and α = ln2 / Tc. BrdU is incorporated into DNA strand instead of TdR. Anti BrdU monoclonal antibody is used to distinguish labeled and unlabeled cells in a population and to determine the fraction. The plotting of labeled or unlabeled cell fraction against time reveals the cell cycle time.</p>

収録刊行物

  • Cytometry research = 日本サイトメトリー学会機関誌

    Cytometry research = 日本サイトメトリー学会機関誌 16(2), 37-41, 2006-09-25

    日本サイトメトリー学会

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