Cell Cycle Analysis using Flow Cytometry II

• Fujikawa-Yamamoto Kohzaburo

  Division of Cell Medicine, Research Institute of Medical Science, Kanazawa Medical University

• Miyagoshi Minoru

  Division of Cell Medicine, Research Institute of Medical Science, Kanazawa Medical University

Bibliographic Information

Other Title

• フローサイトメトリ－を用いた細胞周期解析Ⅱ
• ―BrdU 連続ラベル法と細胞周期時間―
• - BrdU Continuous Labeling and Cell Cycle Time -

Abstract

The BrdU continuous labeling method was presented for a method that can determine the cell cycle time. In this method, the relationships are derived using an equation that n( t,τ) =αN₀exp(αt) exp (ατ), where n (t,τ) is cell density of a phase τ at time t, N₀ is the initial cell number at time t = 0, Tc is cell-cycle time and α = ln2 / Tc. BrdU is incorporated into DNA strand instead of TdR. Anti BrdU monoclonal antibody is used to distinguish labeled and unlabeled cells in a population and to determine the fraction. The plotting of labeled or unlabeled cell fraction against time reveals the cell cycle time.

Journal

• Cytometry Research

  Cytometry Research 16 (2), 37-41, 2006

  Japan Cytometry Society

Keywords

• Flow cytometry
• Cell cycle analysis
• Cell cycle time
• BrdU

Details

• CRID: 1390282679449057536
• NII Article ID: 10018775177
• NII Book ID: AN10356261
• DOI: 10.18947/cytometryresearch.16.2_37
• ISSN: 24240664
• ISSN: 09166920
• Web Site: https://search.jamas.or.jp/link/ui/2007022037
• Text Lang: ja
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed