近接場光と半導体微小粒子を用いたバイオイメージング Imaging of Cells and Biological Molecules with Total Internal Reflection Fluorescence Microcopy

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著者

    • 辰巳 仁史 TATSUMI Hitoshi
    • 名古屋大学大学院 医学研究科 イメージング生理学 Department of Physiology, Nagoya University Graduate School of Medicine

抄録

One of the major challenges in cell physiology is revealing the molecular mechanism underlying cell functions. This paper reviews applications of near field optics and quantum dots to the cell biology. In the first part (1) imaging of single ion channels with near field illumination and in the following part (2) the process of stress fiber formation examined with quantum dots are introduced.(1) The mechanosensitive channel of large conductance (Msc L) has been examined to explore the molecular basis of the channel gating. We have developed a noble experimental setup; the Msc L is imaged with fluorescent molecular probes for FRET pair, and observed by total internal reflection microscopy.(2) The stress fiber is a long linear structure and orients along the long axis of a cell. We have examined the process of stress fiber formation by employing digitoninpermeabilized semi-intact cells and quantum dots (Qdots).

収録刊行物

  • レーザー研究  

    レーザー研究 34(12), 822-827, 2006-12-15 

    The Laser Society of Japan

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各種コード

  • NII論文ID(NAID)
    10018792506
  • NII書誌ID(NCID)
    AN00255326
  • 本文言語コード
    JPN
  • 資料種別
    REV
  • ISSN
    03870200
  • NDL 記事登録ID
    8619750
  • NDL 雑誌分類
    ZN33(科学技術--電気工学・電気機械工業--電子工学・電気通信)
  • NDL 請求記号
    Z16-1040
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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