フェムト秒レーザー励起パルスの波形整形による2光子励起蛍光顕微計測の高性能化 Highly Functional Two-Photon Excited Fluorescence Microscopy with Pulse Shaping of Femtosecond Excitation Pulses
Femtosecond laser pulses used as a pumping pulse in two-photon excited fluorescence microscopy were adaptively shaped by two separate self-learning schemes. First, the laser pulse was delivered through a singlemode fiber, and its pulse s ape was adaptively optimized before the fiber propagation so that its fluorescent efficiency reaches the highest level. Second, the laser pulse was delivered through a microstructure fiber, and the spectrum shape of the super-continuum generated by the microstructure fiber was adaptively optimized by shaping the input pulse so that the intensity ratio of fluorescence generated from a protein mixture becomes the highest.
レーザー研究 34(12), 833-837, 2006-12-15
The Laser Society of Japan