Specific-detection of alkaline phosphatase activity in individual species of marine phytoplankton

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著者

    • Yamaguchi Haruo YAMAGUCHI HARUO
    • Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency
    • Yamaguchi Mineo YAMAGUCHI MINEO
    • Harmful Phytoplankton Section, Harmful Algal Bloom Division, National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency

抄録

We evaluated the suitability of ELF-based assay (ELF: Enzyme-Labeled Fluorescence, Molecular Probes) as a tool for specific-detection of alkaline phosphatase (AP) activity in individual species of marine phytoplankton. Among 4 species of marine phytoplankton tested in the present culture experiments, the ELF-labeled cells of Karenia mikimotoi (Dinophyceae) and Skeletonema costatum (Bacillariophyceae) were simultaneously found with AP activity that was determined by using 4-methylumbelliferyl phosphate under the phosphorus-starved state but not found under the phosphorus-replete state. There were significant positive correlations between the percentage of ELF-labeled cells and AP activity of K. mikimotoi and of S. costatum. In contrast for Chattonella antiqua and Heterosigma akashiwo (Raphidophyceae) which do not produce AP, no ELF-labeled cells were found even under the phosphorus-starved state. Thus, ELF-based assay is considered to be useful for specific-detection of AP activity.

収録刊行物

  • Plankton & benthos research  

    Plankton & benthos research 1(4), 214-217, 2006-11-01 

    日本プランクトン学会

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各種コード

  • NII論文ID(NAID)
    10018808716
  • NII書誌ID(NCID)
    AA12130745
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    18808247
  • NDL 記事登録ID
    8567823
  • NDL 雑誌分類
    ZR1(科学技術--生物学)
  • NDL 請求記号
    Z54-J424
  • データ提供元
    CJP書誌  CJP引用  NDL  IR  J-STAGE 
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