Spectrofluorometric Determination of Kynurenic Acid with Horseradish Peroxidase in the Presence of Hydrogen Peroxide

DOI PDF Web Site Web Site Web Site 被引用文献3件 参考文献33件
  • ODO Junichi
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science
  • FUNAI Tomomi
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science
  • HIRAI Akihito
    Department of Biological Chemistry, Faculty of Science, Okayama University of Science

この論文をさがす

抄録

The catalytic activity of horseradish peroxidase (HRP) in the presence of hydrogen peroxide has been investigated for the fluorescent derivatization of kynurenic acid under conditions with no exposure to light. Non-fluorescent kynurenic acid was converted into a fluorescent compound (Ex: 367 nm, Em: 470 nm) with HRP in the presence of hydrogen peroxide, and the optimum conditions of this fluorescent derivatization were investigated. Moreover, this fluorescent derivatization was developed for a spectrofluorometric determination of trace amounts of kynurenic acid by measuring the fluorescence intensity of the fluorescent compound. The calibration curve obtained was linear from 1.0 to 10.0 nmol of kynurenic acid in a 1.0 mL sample solution. The relative standard deviation at 5.0 nmol of kynurenic acid was 5.71% (n = 5). By adjusting the bandwidths for both the excitation and emission to 15 nm, the calibration curve was also linear in the range between 0.1 to 1.0 nmol of kynurenic acid in a 1.0 mL sample solution. This method was applied to the fluorometric determination of trace amounts of kynurenic acid in the control sera.

収録刊行物

  • Analytical Sciences

    Analytical Sciences 23 (3), 317-320, 2007

    社団法人 日本分析化学会

被引用文献 (3)*注記

もっと見る

参考文献 (33)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ