Time-Resolved Fluoroimmunoassay for 19-Nortestosterone Residues in Aquaculture Tissues

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  • LI Xiangqian
    Department of Chemical and Bioengineering, Huaiyin Institute of Technology
  • HUO Tieming
    School of Food Science and Technology, Southern Yangtze University
  • CHU Xiaogang
    School of Food Science and Technology, Southern Yangtze University
  • XU Chuanlai
    School of Food Science and Technology, Southern Yangtze University

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抄録

A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of 19-nortestosterone (17β-NT) residues in aquaculture tissues. The limit of detection (LOD) was determined to be 0.08 ng g-1 and the limit of quantification (LOQ) was less than 0.8 ng g-1. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred aquaculture tissues and confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS). This proposed technique could be applied to routine residue analysis.

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  • Analytical Sciences

    Analytical Sciences 23 (3), 321-325, 2007

    社団法人 日本分析化学会

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