Determination of Lysozyme at the Nanogram Level by a Resonance Light-Scattering Technique with Functionalized CdTe Nanoparticles

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  • LI Juan
    Department of Chemistry, Nankai University
  • HE Xi-Wen
    Department of Chemistry, Nankai University
  • WU Yun-Li
    Department of Chemistry, Nankai University
  • LI Wen-You
    Department of Chemistry, Nankai University
  • ZHANG Yu-Kui
    Department of Chemistry, Nankai University National Chromatographic Research & Analysis Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences

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Abstract

Nanoparticles of cadmium telluride coated with mercaptoacetic acid were prepared in the water phase. Further, an assay of lysozyme with a sensitivity at the nanogram level is proposed. At pH 7.28, lysozyme with positive charges can interact with CdTe nanoparticles. The resonance light-scattering (RLS) signals of functionalized nano-CdTe were greatly enhanced by lysozyme in the region of 300 - 600 nm, characterized with peaks located at 367, 470 and 533 nm. A linear relationship could be established between the enhanced RLS intensity and the lysozyme concentration in the range of 0.06 - 4.0 µg mL-1. The limit of detection was 9.5 ng mL-1. The contents of lysozyme were determined with recoveries of 95.6 - 104.8% and RSD of 1.5 - 2.3%, respectively. This method is sensitive, rapid, accurate and simple, and provides a new and reliable means for the quantity determination of lysozyme.

Journal

  • Analytical Sciences

    Analytical Sciences 23 (3), 331-335, 2007

    The Japan Society for Analytical Chemistry

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