書誌事項
- タイトル別名
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- Possibility and problems of the micro-array technique and cluster analysis in the research on oral carcinoma
- コウクウガン ケンキュウ ニ オケル マイクロアレイ ギジュツ ト クラスター カイセキ ノ カノウセイ ト カダイ
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抄録
Recently, comprehensive studies of gene expression in cancer have been made possible by the micro-array technique. However, such studies have not always yielded a comprehensive understanding of genetic phenomena in cancer and too much data may confuse researchers, In searching for the genes responsible for tumor metastasis and/or cisplatin resistance, we have demonstrated micro-array analysis combined with gene cluster analysis. During our studies, we faced difficulties such as the precise amplification of micro-scale mRNA, quality check of mRNA, and control of the labeling difference between Cy3- and Cy5-dUTP. In order to solve these problems, we checked RNA by electrophoresis, optical meters, and Affymetrix Test ChipTM, and confirmed the array data by quantitative real-time PCR. In the cluster analysis, contamination of poor data and hidden common characters among the samples were critical problems. Pathway analysis and evaluation of the gene function using clinical cases and/or transformation were useful to help detect candidate gene clusters.
収録刊行物
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- 日本口腔科学会雑誌
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日本口腔科学会雑誌 55 (2), 95-99, 2006
特定非営利活動法人 日本口腔科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206434896512
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- NII論文ID
- 130004335931
- 10018827324
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- NII書誌ID
- AN00189050
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- ISSN
- 21850461
- 00290297
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- NDL書誌ID
- 7924294
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- NDL
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可