Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1

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著者

    • TANAKA Katsuyuki
    • Department of Biological Science, Graduate School of Science, Hiroshima University
    • URBANCZYK Henryk
    • Department of Biological Science, Graduate School of Science, Hiroshima University
    • MATSUI Hiroki
    • Department of Biological Science, Graduate School of Science, Hiroshima University
    • SUZUKI Katsunori
    • Department of Biological Science, Graduate School of Science, Hiroshima University

抄録

Most plant pathogenic <i>Agrobacterium</i> strains have been classified into three biovars, "biovar 1 (<i>A. tumefaciens</i>; <i>Rhizobium radiobacter</i>), biovar 2 (<i>A. rhizogenes</i>; <i>R. rhizogenes</i>) and biovar 3 (<i>A. vitis</i>; <i>R. vitis</i>)". The bacteria possess diverse types of genomic organization depending on the biovar. Previous genomic physical maps indicated difference in location of rDNA and chromosomally-coded virulence genes between biovar 1 and 2 genomes. In order to understand biovar 3 genome and its evolution in relation to the biovar 1, 2 and 3 genomes, we constructed physical map of a pathogenic biovar 3 strain K-Ag-1 in this study. Its genome consisted of two circular chromosomes (3.6 and 1.1 Mbp in length), and three plasmids (560, 230 and 70 kbp). Gene mapping based on the physical map showed presence of two rDNA loci in the larger chromosome and at least one rDNA locus in the smaller chromosome. Six chromosomal virulence genes, namely <i>chvA</i>, <i>chvD</i>, <i>chvE</i>, <i>glgP</i>, <i>exoC</i> and <i>ros</i> were found in the larger chromosome and not in the smaller chromosome. The location of rDNA loci is similar with that of biovar 1 genome, whereas the location of chromosomal virulence genes is similar with that of biovar 2 genome despite of the closer 16S-rRNA based phylogenetic relation of biovar 3 with biovar 1 than with biovar 2. Genomic PFGE RFLP analysis revealed that the K-Ag-1 strain, which was isolated on a kiwifruit plant in Japan, has the closest intra-species relation with two strains isolated from grapevine plants in Japan among eight biovar 3 strains examined. This datum suggests that the line of the strain is a major one in biovar 3 in Japan. Evolution of the genome of the strain is discussed based on the data.<br>

収録刊行物

  • Genes & genetic systems

    Genes & genetic systems 81(6), 373-380, 2006-12-25

    The Genetics Society of Japan

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各種コード

  • NII論文ID(NAID)
    10018827943
  • NII書誌ID(NCID)
    AA11077421
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13417568
  • データ提供元
    CJP書誌  J-STAGE 
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