Formation of Zinc Protoporphyrin IX and Protoporphyrin IX from Oxymyoglobin in Porcine Heart Mitochondria

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著者

    • ISHIKAWA Hiroya
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University
    • KAWABUCHI Taiki
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University
    • KAWAKAMI Yuriko
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University
    • MATSUMOTO Kiyoshi
    • Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School of Kyushu University

抄録

The formation of zinc protoporphyrin IX (ZnPP) and protoporphyrin IX (PP) in porcine heart mitochondria was investigated by fluorescent spectral analysis. The time-course study at 37°C and pH 5.5 with 100μM ZnCl<SUB>2</SUB> showed a linear formation of ZnPP from oxymyoglobin after a lag-period (9h) at the formation rate of 0.46nmol/h. The formed amount of ZnPP is dependent upon the ZnCl<SUB>2</SUB> concentration. In the incubation without ZnCl<SUB>2</SUB>, the new peak derived from PP appeared at 633nm after 12h, and then its intensity gradually increased. The formation of PP is believed to be due to the release of Fe<SUP>2+</SUP> in porphyrin ring of oxymyoglobin during the incubation. This suggests that the mitochondria have the ability to form PP from oxymyoglobin, and therefore, are directly related to the release of Fe<SUP>2+</SUP> from porphyrin ring in myoglobin.

収録刊行物

  • Food science and technology research  

    Food science and technology research 13(1), 85-88, 2007-02-01 

    Japanese Society for Food Science and Technology

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各種コード

  • NII論文ID(NAID)
    10018852469
  • NII書誌ID(NCID)
    AA11320122
  • 本文言語コード
    ENG
  • 資料種別
    NOT
  • ISSN
    13446606
  • データ提供元
    CJP書誌  CJP引用  J-STAGE 
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