口腔扁平上皮癌細胞の浸潤能に及ぼすRNAiを用いたets-1遺伝子発現抑制の影響 : ets-1遺伝子の機能評価 Effect of RNAi-induced inhibition of ets-1 gene expression on invasion properties of oral squamous carcinoma cells : Functional evaluation of ets-1 gene
Metastasis in invasive carcinoma is closely associated with the production of extracellular matrix catabolic enzymes, such as matrix metalloproteinases (MMPs). It is known that<I>ets</I>proto-oncogenes exist in transcriptional regulation refions of the MMP gene and enhance or inhibit MMP transcription. In this study, we restrained the<I>ets</I>-1 gene expression of an oral squamous carcinoma cell line by means of RNA interference (RNAi) and examined the change in invasion ability from a molecular biological perspective. An oral squamous carcinoma cell line (HSC-3) was transfected with short interfering RNA (siRNA) of<I>ets</I>-1. The invasive ability of the transfected cells was determined by a matrigel invasion assay, and MMP-9 production was measured by gelatin zymography. These data were compared with those of normal cultured cells. Matrigel invasion assay demonstrated that the transfected cells had a lower invasive ability than HSC-3, and gelatin zymography demonstrated that the MMP-9 production in these cells had decreased. These results suggested that transfection of siRNA<I>ets</I>-1 would inhibit tumor invasion through down-regulation of MMP-9.
日本口腔外科学会雑誌 53(1), 2-7, 2007-01-20
Japanese Society of Oral and Maxillofacial Surgeons