口腔扁平上皮癌細胞の浸潤能に及ぼすRNAiを用いたets-1遺伝子発現抑制の影響 : ets-1遺伝子の機能評価 Effect of RNAi-induced inhibition of ets-1 gene expression on invasion properties of oral squamous carcinoma cells : Functional evaluation of ets-1 gene

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著者

    • 大西 祐一 OHNISHI Yuichi
    • 大阪歯科大学口腔外科学第二講座 Second Department of Oral and Maxillofacial Surgery, Osaka Dental University
    • 覚道 健治 KAKUDO Kenji
    • 大阪歯科大学口腔外科学第二講座 Second Department of Oral and Maxillofacial Surgery, Osaka Dental University

抄録

Metastasis in invasive carcinoma is closely associated with the production of extracellular matrix catabolic enzymes, such as matrix metalloproteinases (MMPs). It is known that<I>ets</I>proto-oncogenes exist in transcriptional regulation refions of the MMP gene and enhance or inhibit MMP transcription. In this study, we restrained the<I>ets</I>-1 gene expression of an oral squamous carcinoma cell line by means of RNA interference (RNAi) and examined the change in invasion ability from a molecular biological perspective. An oral squamous carcinoma cell line (HSC-3) was transfected with short interfering RNA (siRNA) of<I>ets</I>-1. The invasive ability of the transfected cells was determined by a matrigel invasion assay, and MMP-9 production was measured by gelatin zymography. These data were compared with those of normal cultured cells. Matrigel invasion assay demonstrated that the transfected cells had a lower invasive ability than HSC-3, and gelatin zymography demonstrated that the MMP-9 production in these cells had decreased. These results suggested that transfection of siRNA<I>ets</I>-1 would inhibit tumor invasion through down-regulation of MMP-9.

収録刊行物

  • 日本口腔外科学会雑誌  

    日本口腔外科学会雑誌 53(1), 2-7, 2007-01-20 

    Japanese Society of Oral and Maxillofacial Surgeons

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各種コード

  • NII論文ID(NAID)
    10018858475
  • NII書誌ID(NCID)
    AN00189163
  • 本文言語コード
    JPN
  • 資料種別
    ART
  • ISSN
    00215163
  • NDL 記事登録ID
    8659025
  • NDL 雑誌分類
    ZS44(科学技術--医学--歯科学・口腔外科学)
  • NDL 請求記号
    Z19-145
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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